CST, 14773S, SYVN1 (D3O2A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SYVN1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SYVN1 (D3O2A) Rabbit Monoclonal Antibody (CST #14773) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SYVN1 (D3O2A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SYVN1 protein. This antibody does not cross-react with AMFR protein, but may cross-react with an unidentified protein of 60 kDa. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SYVN1 protein. Background Synoviolin-1 (SYVN1/HRD1) is a RING-type E3 ubiquitin-protein ligase and major component of the endoplasmic reticulum (ER) quality control system that is involved in the ubiquitin-dependent degradation of misfolded proteins (1). SYVN1 is a multispanning ER membrane protein whose expression is upregulated at the protein level under conditions that promote ER stress (1-4). Research studies have shown that SYVN1 is an anti-apoptotic factor that is implicated in the pathogenesis of arthropathy by promoting synovial hyperplasia (5). Furthermore, gene-targeting studies have demonstrated that SYVN1 expression is indispensable for embryogenesis (6). Alternate Names DER3; E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog; HRD1; KIAA1810; MGC40372; RING-type E3 ubiquitin transferase synoviolin; Synovial apoptosis inhibitor 1; synovial apoptosis inhibitor 1, synoviolin; synoviolin 1; SYVN1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG