CST, 14998S, MTHFD1L (D7O8E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MTHFD1L. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MTHFD1L (D7O8E) Rabbit Monoclonal Antibody (CST #14998) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MTHFD1L (D7O8E) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTHFD1L protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro52 of human MTHFD1L protein. Background NADP+ dependent methylenetetrahydrofolate dehydrogenase 1-like (MTHFD1L) is a mitochondrial enzyme that catalyzes the production of formate from 10-formyl-tetrahydrofolate, the last step in one-carbon (1-C) flow from mitochondria to cytoplasm (1,2). These one-carbon end products are required for synthesis of thymidylate and purines. In the mitochondria, these essential one-carbon products are formed by a series of reactions catalyzed by a pair of enzymes (MTHFD2 and MTHFD1L), but by the trifunctional MTHFD1 enzyme in the cytoplasm (3). The 10-formyl-tetrahydrofolate synthetase MTHFD1L is widely expressed in most adult tissues and at all stages of mammalian embryonic development (1). Research studies using MTHFD1L knockout mice indicate that MTHFD1L plays an essential role in neural tube formation; mice lacking MTHFD1L displayed neural tube and craniofacial defects leading to embryonic lethality (4). Alternate Names 10-formyl-THF synthetase; C1TM; dJ292B18.2; DKFZp586G1517; FLJ21145; Formyltetrahydrofolate synthetase; formyltetrahydrofolate synthetase domain containing 1; FTHFSDC1; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like; mitochondrial C1-tetrahydrofolate synthase; mitochondrial C1-tetrahydrofolate synthetase; Monofunctional C1-tetrahydrofolate synthase, mitochondrial; MTC1THFS; MTHFD1L Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 106 Source/Isotype: Rabbit IgG