CST, 15125T, BRD7 (D9K2T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BRD7. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, BRD7 (D9K2T) Rabbit Monoclonal Antibody (CST #15125) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity BRD7 (D9K2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total BRD7 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human BRD7 protein. Background Bromodomain-containing protein 7 (BRD7, BP75, CELTIX-1) is a conserved bromodomain-containing protein that was first identified in a screen for proteins that interact with the PDZ domain of PSD95 (1). Subsequent studies identified BRD7 as a major component of SWI/SNF chromatin remodeling complexes, where it was shown to interact directly with acetylated histones to regulate gene transcription (2,3). BRD7 also interacts with p53, and was shown to participate directly in p53-dependent transcriptional regulation (4). Loss-of-function BRD7 mutations were identified in a subset of wild-type p53 breast cancer tumor samples, implicating BRD7 as a putative tumor-suppressor of potential clinical significance (5). BRD7 also associates with the BRCA1 protein, an interaction that facilitates recruitment of BRCA1 to the ERα gene promoter (6). Alternate Names 75 kDa bromodomain protein; BP75; BRD7; bromodomain containing 7; bromodomain protein; bromodomain-containing 7; Bromodomain-containing protein 7; CELTIX1; NAG4; Protein CELTIX-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG