CST, 15416S, CD80 (E3Q9V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD80. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD80 (E3Q9V) Rabbit Monoclonal Antibody (CST #15416) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD80 (E3Q9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD80 protein. This antibody detects a 30 kDa band of unknown origin in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human CD80 recombinant protein. Background CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9). Alternate Names Activation B7-1 antigen; B-lymphocyte activation antigen B7; B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-75 Source/Isotype: Rabbit IgG