CST, 15571S, TFF1/pS2 (D2Y1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TFF1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TFF1/pS2 (D2Y1J) Rabbit Monoclonal Antibody (CST #15571) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:150 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 72033 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity TFF1/pS2 (D2Y1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total TFF1/pS2 protein. This antibody does not cross-react with either TFF2 or TFF3 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val33 of human TFF1/pS2 protein. Background The trefoil factor (TFF) family of proteins (TFF1/pS2, TFF2, and TFF3) are a group of highly conserved, secreted polypeptides that are expressed by mucus-secreting cells of the gastrointestinal tract. Within the gastrointestinal tract, TFFs display both common and distinct expression patterns (1). Collectively, the TFF family of proteins play a prominant role in the protection and repair of the mucous epithelia lining the gastrointestinal tract through their interactions with mucins (2). TFFs have been shown to regulate a number of cellular processes such as migration, apoptosis, and proliferation. In humans, dysregulated expression of TFFs has been observed in inflammatory bowel diseases as well as tumors of the breast, colon, lung, and stomach (2). TFF1/pS2 is a gastric peptide that is highly expressed by mucosal goblet cells of the stomach, where it is thought to play a role in maintaining the integrity of the epithelial layer of the mucosa through the regulation of cell-cell adhesion and cell migration (2,3). Research studies have shown that TFF1 functions as a tumor suppressor in the stomach as its expression is frequently lost in gastric carcinomas, largely due to promoter hypermethylation (4-8). Research studies have also demonstrated that is a transcriptional target of estrogen receptor-α and that TFF1 expression in breast carcinoma may be used as a predictive biomarker for response to anti-estrogen therapy (9,10). Alternate Names BCEI; Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; HP1.A; HPS2; P04155; pNR-2; Polypeptide P1.A; Protein pS2; pS2; TFF1; Trefoil factor 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG