CST, 15627T, TUT4 (E9D9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZCCHC11. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TUT4 (E9D9T) Rabbit Monoclonal Antibody (CST #15627) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TUT4 (E9D9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total TUT4 protein. This antibody recognizes 82 kDa and 65 kDa bands of unknown origin. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His12 of human TUT4 protein. Background Terminal uridylyl transferase 4 and 7 (TUT4/7), also known as ZCCHC11 and ZCCHC6 respectively, are enzymes that posttranscriptionally uridylate mRNAs and miRNAs at the 3' end (1,2). The TUT enzymes can monouridylate double-stranded RNA with a single nucleotide overhang, which in turn creates a recognition site for Dicer to process miRNAs (3). Many of the miRNAs recognized by TUT4/7 are involved in cell differentiation such as the gene cluster (4). TUT4 and TUT7 have been shown to be recruited by Lin28 in stem cells to uridylate and block expression of let-7 miRNA, a microRNA with tumor suppressive qualities (3,5,6). TUT4 and TUT7 play a role in the mRNA decay pathway, where they polyuridylate targeted mRNAs to promote turnover (7). TUT4 and TUT7 have also been implicated in T cell activation, innate immune response, and antiviral defenses (8-10). Both TUT proteins have also been shown to prevent retrotransposition of LINE-1 elements, indicating a role in maintaining genomic stability (11). Alternate Names KIAA0191; PAP associated domain containing 3; PAPD3; TENT3A; terminal uridylyl transferase 4; Terminal uridylyltransferase 4; TUT4; TUTase 4; ZCCHC11; Zinc finger CCHC domain-containing protein 11; zinc finger CCHC-type containing 11; zinc finger, CCHC domain containing 11 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 205 Source/Isotype: Rabbit IgG