CST, 16212S, PRDM16 (F3K9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRDM16. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PRDM16 (F3K9T) Rabbit Monoclonal Antibody (CST #16212) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PRDM16 (F3K9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRDM16 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro666 of human PRDM16 protein. Background PRDM16 is a transcription factor that is structurally characterized by a conserved N-terminal PRDI-BF1 and RIZ1 homology (PR) domain and a variable number of zinc fingers (1,2). The PR domain bears similarities to the catalytic suppressor of variegation 3-9, enhancer of zeste, and trithorax (SET) domain that is found in a group of histone methyltransferases (1,3). Indeed, PRDM16 has been shown to catalyze mono-methylation of histone 3 at lysine (H3K9me1) (4). Human PRDM16 encodes a protein with four isoforms with the most extensively studied isoforms being the full-length PRDM16 and the PR-lacking isoform that is generated by a transcript derived from an internal promoter (5). PRDM16 is involved in the regulation of various biological processes, including adipose biology and energy homeostasis. This protein is also expressed in hematopoietic stem cells (HSCs) and plays a key role in the establishment and maintenance of these cells (6,7). More recently, PRDM16 was shown to be a suppressor of lung adenocarcinoma metastasis, and its low expression predicted poor prognosis of lung adenocarcinoma patients (8). Alternate Names CMD1LL; Histone-lysine N-methyltransferase PRDM16; KIAA1675; KMT8F; LVNC8; MDS1/EVI1-like gene 1; MEL1; MGC166915; PFM13; PR domain 16; PR domain containing 16; PR domain zinc finger protein 16; PR domain-containing protein 16; PR-domain zinc finger protein 16; PR/SET domain 16; PRD16; PRDM16; Transcription factor MEL1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 170 Source/Isotype: Rabbit IgG