CST, 16431S, BCAT1 (D4V2T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BCAT1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, BCAT1 (D4V2T) Rabbit Monoclonal Antibody (CST #16431) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BCAT1 (D4V2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total BCAT1 protein. This antibody does not cross-react with BCAT2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCAT1 protein. Background BCAT1 and BCAT2 are cytosolic and mitochondrial branched chain aminotransferases, respectively (1,2). Research studies have implicated BCAT1 in distant metastasis in patients with advanced colorectal cancer (3). Disruption of BCAT2 in mice leads to higher levels of plasma branched-chain amino acids, reduced adiposity and body weight, and increased energy expenditure, suggesting its role in regulating insulin sensitivity (4). Alternate Names BCAT(c); BCAT1; BCATC; BCT1; branched chain amino acid transaminase 1; branched chain amino-acid transaminase 1, cytosolic; branched chain aminotransferase 1, cytosolic; Branched-chain-amino-acid aminotransferase, cytosolic; DKFZp686E12175; ECA39; MECA39; placental protein 18; PNAS-121; PNAS121; PP18; Protein ECA39 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG