CST, 16743S, Orexin (D6G9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HCRT. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Orexin (D6G9T) Rabbit Monoclonal Antibody (CST #16743) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Frozen): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Orexin (D6G9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total orexin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the sequence of the mature human orexin protein. Background Orexin, also called HCRT or hypocretin, is expressed as a precursor that is processed into two biologically active neuropeptides called orexin-A (HCRT1) and orexin-B (HCRT2) (1). Orexin is expressed in the lateral and posterior hypothalamus and plays a role in feeding behavior (1). The orexin neuropeptides act by stimulating the two orexin receptors that belong to the G Protein-Coupled Receptors family (1). Orexin receptor 1 is mostly found in the hypothalamic region, while orexin receptor 2 is also found in the cerebral cortex and the nucleus accumbent (2). Orexin also plays a role in adipocyte homeostasis and is required for brown adipose tissue development, differentiation, and function (3). Alternate Names HCRT; Hcrt1; Hcrt2; hypocretin; hypocretin (orexin) neuropeptide; hypocretin (orexin) neuropeptide precursor; Hypocretin neuropeptide precursor; Hypocretin-1; Hypocretin-2; NRCLP1; OREX; Orexin; Orexin-A; Orexin-B; OX; PPORX; PPOX; prepro-orexin Specification REACTIVITY: H M R SENSITIVITY: Endogenous (IHC-P, IF-F), Transfected (W) MW (kDa): 16 Source/Isotype: Rabbit IgG