CST, 16960T, SIX1 (D5S2S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SIX1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SIX1 (D5S2S) Rabbit Monoclonal Antibody (CST #16960) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73583 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SIX1 (D5S2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total SIX1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp269 of human SIX1 protein. Background Sine oculis homeobox (SIX) proteins belong to a family of evolutionarily conserved transcription factors discovered in mutant screens for embryonic eye development genes (1-3). The prototypical family member ( , ) was named for eyeless embryos carrying mutations in a gene highly conserved among vertebrates, including humans ( ) (4). A total of six family members (SIX1-6) have been identified in vertebrates. Each SIX protein contains a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions (5). In addition to their critical functions during embryonic organogenesis, research studies suggest that SIX proteins play additional roles in postnatal cell cycle regulation, with potentially important implications in tumorigenesis (6,7). In contrast to the ortholog, the vertebrate gene product does not play a critical role in embryonic eye development. Vertebrate SIX1 is required for development of mesoderm- and neural crest-derived lineages, and male reproductive tissues (8-10). SIX1 has also been shown to regulate transcription of MyoD in adult muscle progenitor cells during postnatal muscle development (11). A mechanistic role for SIX1 in cell cycle regulation is supported by research studies showing increased SIX1 expression in various cancer subtypes, including breast, ovarian, and hepatocellular carcinoma (6,12,13). Alternate Names BOS3; DFNA23; Homeobox protein SIX1; Sine oculis homeobox homolog 1; SIX homeobox 1; SIX1; TIP39 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 36 Source/Isotype: Rabbit IgG