CST, 16984S, CAR (D3W3G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CAR. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CAR (D3W3G) Rabbit Monoclonal Antibody (CST #16984) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:500 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 74159 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CAR (D3W3G) Rabbit Monoclonal Antibody recognizes endogenous levels of total CAR protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg328 of human CAR protein. Background The coxsackie virus and adenovirus receptor (CXADR, CAR) is a highly conserved, single-transmembrane glycoprotein and the primary receptor to mediate cellular attachment and infection of coxsackie B viruses and most adenoviruses (1,2). The CAR protein contains a pair of Ig-like domains within the amino-terminal extracellular domain and a carboxyl-terminal PDZ motif (1). Research studies indicate that CAR is a tight junction protein that associates with the ZO-1 scaffold protein and promotes both cell adhesion and restriction of solute and ion movement between cells (2). Endogenous CAR is targeted to the basolateral plasma membrane by a tyrosine-based basolateral sorting signal and clathrin adaptors AP-1A and AP-1B (3). CAR binds junctional adhesion molecule L (JAML) on epithelial cells and neutrophils where it activates PI3K and downstream MAPK kinases to stimulate epithelial γδ T cell proliferation and increase production of TNFα and keratinocyte growth factor (4-6). As a result, the CAR protein plays a potentially critical role in adenoviral gene therapy, immunity, wound repair, inflammation, and cancer therapy (4-6). Additional studies demonstrate that CAR is essential in regulating squamous carcinoma cell growth (7). Alternate Names 46 kD coxsackievirus and adenovirus receptor (CAR) protein; CAR; CAR4/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; Coxsackievirus and adenovirus receptor; Coxsackievirus B-adenovirus receptor; CVB3 binding protein; CVB3-binding protein; CXADR; CXADR Ig-like cell adhesion molecule; CXAR; hCAR; HCVADR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-55 Source/Isotype: Rabbit IgG