CST, 17673S, UBE4B (F4F3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UBE4B. Validated for WB,WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, UBE4B (F4F3K) Rabbit Monoclonal Antibody (CST #17673) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity UBE4B (F4F3K) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBE4B protein. This antibody detects a 75 kDa and a 90 kDa protein of unknown identity in some cell lines. Nonspecific labeling of fixed frozen mouse colon and kidney have been observed by immunofluorescence. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro62 of human UBE4B protein. Background Ubiquitination Factor E4B (UBE4B) is a mammalian homolog of the protein UFD2 and the first identified E4 ubiquitination factor (1). As a component of the ubiquitin-proteasome system (UPS), UBE4B functions as both an E3 and E4 ubiquitin ligase and is involved in various cellular processes, including DNA damage response, cell cycle regulation, protein quality control, and signal transduction. Its key role includes facilitating the polyubiquitination of specific substrates, most notably the tumor suppressor p53, thereby promoting its MDM2-mediated proteasomal degradation (2). Overexpression or gene amplification of UBE4B has been observed in brain tumors, breast cancer, and hepatocellular carcinoma, where inhibition of p53 leads to decreased apoptosis and increased tumor cell survival (3-5). Recent studies have shown that UBE4B phosphorylation through ATR signaling reduces its binding affinity to p53, whereas dephosphorylation by Wip1 plays a crucial role in stabilizing the activity of UBE4B in response to DNA damage (6). UBE4B also plays a role in neuronal survival and death regulation, and is associated with pathways independent of the p53 family, such as polyglutamine aggregation and Wallerian degeneration (7). It is involved in the polyubiquitination of abnormal proteins that accumulate in neurodegenerative diseases, such as ataxin-3 in Machado-Joseph disease (8), and regulates APP ubiquitination and trafficking, impacting the generation of amyloid β in Alzheimer's disease plaques (9). Additionally, UBE4B enables cytosolic-mitochondrial communication of cellular stress via its translocation to mitochondria and elongation of ubiquitin chains on mitofusin, leading to mitochondrial fragmentation (10). Alternate Names E4; HDNB1; homologous to yeast UFD2; Homozygously deleted in neuroblastoma 1; homozygously deleted in neuroblastoma-1; KIAA0684; RING-type E3 ubiquitin transferase E4 B; UBE4B; Ubiquitin conjugation factor E4 B; Ubiquitin fusion degradation protein 2; ubiquitin-fusion degradation protein 2; ubiquitination factor E4B; ubiquitination factor E4B (homologous to yeast UFD2); ubiquitination factor E4B (UFD2 homolog, yeast); UBOX3; UFD2; UFD2A Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG