CST, 17838S, Notch2 (D76A6) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3060)

Monoclonal Antibody for studying Notch2. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Notch2 (D76A6) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3060) (CST #17838) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Notch2 (D76A6) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3060) recognizes endogenous levels of total Notch2 protein. Species cross-reactivity for IHC-P is human only. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala2378 of human Notch2 protein. Background Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4). Notch2 is a member of the Notch family and mutation in Notch2 is associated with Alagille syndrome (5). Alternate Names AGS2; HJCYS; hN2; N2ECD; N2ICD; Neurogenic locus notch homolog protein 2; NOTC2; Notch 2; Notch 2 extracellular truncation; Notch 2 intracellular domain; Notch homolog 2; Notch homolog 2 (Drosophila); notch receptor 2; NOTCH2 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit