CST, 18035SF, Glut3 (E7M7V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Glut3. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, Glut3 (E7M7V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #18035) is ready to ship. Product Usage Information This product is the carrier free version of product #40538. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 40538 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Glut3 (E7M7V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Glut3 protein. This antibody does not cross-react with Glut1, Glut2, or Glut4. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala472 of human Glut3 protein. Background Glucose transporter 3 (Glut3, also known as SLC2A3) has a higher affinity for glucose than the ubiquitous glucose transporter Glut1. In the normal brain, Glut3 expression is limited to neurons, whereas Glut1 is expressed in both neurons and glia. Glioblastoma-derived brain tumor initiating cells (BTICs) express increased levels of Glut3, enabling their survival in the microenvironment deficient in glucose. In addition, Glut3 expression levels correlate with poor survival in brain tumors (1). Furthermore, integrin αvβ3 was shown to stimulate PAK4-YAP/TAZ signaling to upregulate Glut3 expression in glioblastoma cells. A subpopulation of glioblastoma was identified to be addicted to Glut3 and is highly sensitive to the disruption of integrin αvβ3-PAK4-YAP/TAZ signaling (2,3). Alternate Names FLJ90380; Glucose transporter type 3, brain; GLUT-3; GLUT3; GTR3; SLC2A3; solute carrier family 2 (facilitated glucose transporter), member 3; solute carrier family 2 member 3; Solute carrier family 2, facilitated glucose transporter member 3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-70 Source/Isotype: Rabbit IgG