CST, 18048T, CDK2 (E8J9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cdk2. Validated for WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CDK2 (E8J9T) Rabbit Monoclonal Antibody (CST #18048) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 34541 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CDK2 (E8J9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDK2 protein. In rodent cell lines, isoforms 1 and 2 of CDK2 run as two distinct bands by western blot. This antibody non-specifically labels the muscle in the mouse retina and the vasculature in the mouse brain by immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro294 of human CDK2 protein. Background Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5). Alternate Names cdc2-related protein kinase; CDK2; CDKN2; cell devision kinase 2; Cell division protein kinase 2; cyclin dependent kinase 2; Cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG