CST, 18268S, Granzyme H (E4T2E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Granzyme H. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Granzyme H (E4T2E) Rabbit Monoclonal Antibody (CST #18268) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Granzyme H (E4T2E) Rabbit Monoclonal Antibody recognizes endogenous levels of total Granzyme H protein. This antibody does not cross-react with human Granzyme A, B, K, or M proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln195 of human Granzyme H protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Granzyme H has chymotrypsin-like thioester activity with a preference for hydrophobic, aromatic amino acid residues, such as phenylalanine, tyrosine, or methionine, at the P1 site (4,5). Granzyme H is predominantly expressed at high levels in NK cells, but not in T lymphocytes, and has also been described in mast cells (6-8). After perforin-mediated entry into a target cell, Granzyme H induces many hallmarks of programmed cell death, such as mitochondrial depolarization, generation of reactive oxygen species, DNA degradation, and chromatin condensation (9,10). Alternate Names Cathepsin G-like 2; cathepsin G-like 2, protein h-CCPX; CCP-X; CGL-2; CGL2; CSP-C; CTLA1; CTSGL2; Cytotoxic serine protease C; Cytotoxic T-lymphocyte proteinase; cytotoxic T-lymphocyte-associated serine esterase 1; cytotoxin serine protease-C; GRAH; Granzyme H; granzyme H (cathepsin G-like 2, protein h-CCPX); GZMH Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25-35 Source/Isotype: Rabbit IgG