CST, 18572S, Syntaxin 1A (D4E2W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STX1A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Syntaxin 1A (D4E2W) Rabbit Monoclonal Antibody (CST #18572) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Syntaxin 1A (D4E2W) Rabbit Monoclonal Antibody recognizes endogenous levels of total Syntaxin 1A protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val237 of human Syntaxin 1A protein. Background Syntaxin 1A (STX1A) is a SNARE protein involved in intracellular membrane fusion, including synaptic vesicle fusion (1). At the synapse, syntaxin 1 is located at the presynaptic plasma membrane and is therefore categorized as a t-SNARE protein (2). The amino-terminal domain of syntaxin 1 interacts with Munc18-1 and this interaction is essential for synaptic vesicle fusion (3). Although originally characterized from neural tissues, research studies have demonstrated syntaxin 1A expression in exocrine tissues such as pancreatic islets (4) where it negatively regulates insulin release (5). Alternate Names HPC-1; Neuron-specific antigen HPC-1; P35-1; STX1; STX1A; SYN1A; syntaxin 1A; syntaxin 1A (brain); Syntaxin-1A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG