CST, 18791T, TRMT6 (E7L3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRMT6. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRMT6 (E7L3M) Rabbit Monoclonal Antibody (CST #18791) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRMT6 (E7L3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRMT6 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro333 of human TRMT6 protein. Background TRMT6 and TRMT61A, formerly known as GCD10 and GCD14 in yeast, are essential, conserved proteins that form a heterodimer responsible for N1-methylation (m1A) of adenosine 58 in tRNA (1-4). TRMT6 is an RNA-binding protein that recognizes target substrates, while TRMT61A binds AdoMet to catalyze the methylation event (4). TRMT6/61A can additionally produce the m1A mark in small RNAs such as tRNA-derived fragments (tRFs) and also in mRNAs, though in much lower abundance (5,6). The heterodimeric complex is localized to stress granules during heat shock and provides protection for the transcriptome (7). TRMT6 has shown to play a role in progression of malignant gliomas (8). Disruption of the heterodimer has been shown to negatively affect T cell expansion in mice (9). Alternate Names CGI-09; GCD10; Gcd10p; KIAA1153; MGC5029; mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6; TRM6; TRMT6; tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6; tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6; tRNA methyltransferase 6; tRNA methyltransferase 6 homolog; tRNA methyltransferase 6 homolog (S. cerevisiae); tRNA(m1A58)-methyltransferase subunit TRM6; tRNA(m1A58)MTase subunit TRM6 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG