CST, 18880S, COL4A1 (E5M6R) Rabbit Monoclonal Antibody (IHC Formulated)

Monoclonal Antibody for studying COL4A1. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, COL4A1 (E5M6R) Rabbit Monoclonal Antibody (IHC Formulated) (CST #18880) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24902 . Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity COL4A1 (E5M6R) Rabbit Monoclonal Antibody (IHC Formulated) recognizes endogenous levels of total COL4A1 protein. Non-specific diffuse cytoplasmic staining was observed in skeletal myocytes by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp536 of human COL4A1 protein. Background Type IV collagens support extracellular matrix (ECM) function by forming a cross-linking network in the basement membrane zone. There are six different type IV collagens that are encoded by the following genes: , , , , , and . Among them, COL4A1 and COL4A2 are the most common collagens found in the basement membrane, and are usually used as a marker of the basement membrane dynamic stability (1). The type IV collagen fiber exists as a trimeric complex composed of two molecules of COL4A1 and one molecule of COL4A2. The N-terminal 7S domains of the protein function on intermolecular cross-linking, and C-terminal non-collagenous domains (NCI) are responsible for heterotrimer assembly. The trimeric fiber further associates to form a cross-linked sheet structure by NCI/NCI and 7S/7S interaction (2). COL4A1 plays a critical role in maintaining basement membrane integrity for normal tissue functions. Genetic mutations of COL4A1 and COL4A2 have been linked to cardiovascular disease, cerebral small vessel disease, and glomerulocystic kidney disease (3-5). Alternate Names Arresten; BSVD; BSVD1; CO4A1; COL4A1; COL4A1 NC1 domain; COL4A1s; Collagen 4; Collagen alpha-1(IV) chain; collagen IV; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; collagen type IV; collagen type IV alpha 1 chain; collagen, type IV, alpha 1; PADMAL; RATOR; type IV collagen Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG