CST, 19351SF, Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PABP1 (Arg455/Arg460) a-dimethyl. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #19351) is ready to ship. Product Usage Information This product is the carrier free version of product #3505. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3505 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of PABP1 only when methylated on Arg455 or Arg460. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated. Background Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions, including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined. Alternate Names PAB1; PABP; PABP 1; PABP-1; PABP1; PABPC1; PABPC2; PABPL1; poly(A) binding protein cytoplasmic 1; poly(A) binding protein, cytoplasmic 1; poly(A) binding protein, cytoplasmic 2; Poly(A)-binding protein 1; Polyadenylate-binding protein 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 71 Source/Isotype: Rabbit IgG