CST, 19436S, HELQ (D4K2O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HELQ. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HELQ (D4K2O) Rabbit Monoclonal Antibody (CST #19436) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HELQ (D4K2O) Rabbit Monoclonal Antibody recognizes endogenous levels of total HELQ protein. In some cell lines, this antibody cross-reacts with a 200 kDa band of unknown identity by western blot. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1091 of human HELQ protein. Background DNA interstrand crosslinks (ICLs) are toxic DNA lesions caused by environmental agents as well as some chemotherapeutic drugs. These lesions are repaired via multiple DNA repair pathways. HELQ (also known as HEL308) is a 3'-5' DNA helicase that colocalizes with stalled DNA replication forks in response to DNA damage, and contributes to the repair of ICL lesions through ATR signaling (1,2). HELQ interacts with various DNA repair proteins, including FANCD2, RAD51, RAD51 paralogs, and ATR (1-3). HELQ-deficient mice exhibit reduced fertility as well as interstrand crosslink (ICL) repair defects, and are prone to tumors (3). Alternate Names DNA helicase HEL308; HEL308; Helicase POLQ-like; helicase, POLQ like; helicase, POLQ-like; HELQ; MGC20604; Mus308-like helicase; POLQ-like helicase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG