CST, 19595T, SARS-CoV-2 2'-O-ribose Methyltransferase (E6N2C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying 2'-O-ribose methyltransferase-pp1ab SARS-CoV-2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SARS-CoV-2 2'-O-ribose Methyltransferase (E6N2C) Rabbit Monoclonal Antibody (CST #19595) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 2'-O-ribose Methyltransferase (E6N2C) Rabbit Monoclonal Antibody recognizes transfected levels of total SARS-CoV-2 2'-O-ribose methyltransferase protein. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly39 of SARS-CoV-2 2'-O-ribose methyltransferase protein. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). SARS-CoV-2 2′-O-ribose methyltransferase, or 2′-O-MTase, is a non-structural protein (Nsp16) that is translated from ORF1b. 2′-O-ribose methyltransferase activity along with its co-factor Nsp10, is critically responsible for the formation of a cap on viral RNAs. The viral RNA cap resembles the cap of mRNAs in eukaryotic cells, and provides the viral RNA stability, effective translation, and protection from cellular innate immunity (3). The essential function of this non-structural protein in viral replication has made it an attractive target for potential antiviral strategies (3,4). Alternate Names 2'-O-methyltransferase; 2'-O-ribose methyltransferase; 2'-O-ribose methyltransferase-pp1ab; non-structural protein 16; nsp16; Nsp16 (6799-7096); SARS-CoV-2 nsp16 Specification REACTIVITY: Vir SENSITIVITY: Transfected Only MW (kDa): 40 Source/Isotype: Rabbit IgG