CST, 20008S, CD14 (D1U2Y) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying CD14 mouse. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD14 (D1U2Y) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #20008) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity CD14 (D1U2Y) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp327 of mouse CD14 protein. Background CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage (1). Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein (2). LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection (3-5). Alternate Names Cd14; CD14 antigen; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG