CST, 20099S, BST2 (E4N3W) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying BST2. Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, BST2 (E4N3W) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #20099) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity BST2 (E4N3W) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human BST2 recombinant protein. Background BST2 (CD317, Tetherin, HM1.24) is a type II transmembrane glycoprotein functioning as a major mediator of the innate immune defense against the dissemination of enveloped viruses by tethering virion on the cell surface (1). BST2 has an N-terminal cytoplasmic tail for endocytosis and cytoskeletal signaling, a transmembrane domain, an extracellular domain containing putative disulfide bonds and coiled-coil region for homodimer formation, and a C-terminal GPI domain for membrane anchoring (2,3). Both the transmembrane domain and the GPI domain can insert either into the cell membrane or the viral envelope membrane and hold them together to prevent viral release. Some viruses encode proteins, such as HIV-1 and Vpu, respectively, to act as antagonists to counteract BST2 (2,3). BST2 is overexpressed in gastrointestinal cancers, breast cancer, lung cancer, and multiple myeloma (4-7). BST2 monoclonal antibody targeting myeloma or lung cancer cells induces cellular cytotoxicity and cell death (ADCC, antibody-dependent cell-mediated cytotoxicity). Thus, BST2 serves as a potential target for tumor immunotherapy. Alternate Names Bone marrow stromal antigen 2; bone marrow stromal cell antigen 2; BST-2; BST2; CD317; HM1.24 antigen; NPC-A-7; Tetherin Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG