CST, 20329S, IFIT1 (D2X9Z) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying IFIT1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IFIT1 (D2X9Z) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #20329) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IFIT1 (D2X9Z) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human IFIT1 protein. Background IFIT1 (interferon-induced protein with tetratricopeptide repeats 1) belongs to the IFIT family of proteins, which consists of four members in humans (IFIT1, IFIT2, IFIT3, and IFIT5) and three members in mice (IFIT1, IFIT2, and IFIT3) (1). IFIT1 expression is induced by type I interferons resulting from viral infection (2). IFIT1 is an antiviral protein that directly binds viral RNA that has a 5' triphosphate group (PPP-RNA) (2). In humans, the viral PPP-RNA bound IFIT1 forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent replication (2). IFIT1 has also been shown to inhibit translation by binding to the eukaryotic initiation factor 3 (eIF3) (3). Alternate Names C56; G10P1; GARG-16; IFI-56; IFI-56K; IFI56; IFIT-1; IFIT1; IFNAI1; interferon induced protein with tetratricopeptide repeats 1; Interferon-induced 56 kDa protein; interferon-induced protein 56; Interferon-induced protein with tetratricopeptide repeats 1; interferon-inducible mRNA 561; Interferon, alpha-inducible protein (MW 56kD); ISG56; P56; RNM561 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG