CST, 2051L, Phospho-PKD/PKC mu (Ser916) Antibody

Polyclonal Antibody for studying PKD1/PKCmu (Ser910) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-PKD/PKC mu (Ser916) Antibody (CST #2051) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-PKD/PKC mu (Ser916) Antibody detects endogenous levels of PKD1/PKC mu only when phosphorylated at serine 916. This antibody may also cross-react with isoform PKD2, in some species. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser916 of mouse PKD. Antibodies are purified by protein A and peptide affinity chromatography. Background Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses, such as secretion, gene expression, proliferation, and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6). Alternate Names CHDED; KPCD1; nPKC-D1; nPKC-mu; PKC-MU; PKCM; PKD; PKD1; PRKCM; PRKD1; Protein kinase C mu type; protein kinase C, mu; Protein kinase D; protein kinase D1; Serine/threonine-protein kinase D1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 115 SOURCE: Rabbit