CST, 20584SF, Sox17 (D1T8M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SOX17. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Sox17 (D1T8M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #20584) is ready to ship. Product Usage Information This product is the carrier free version of product #81778. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 81778 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Sox17 (D1T8M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Sox17 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro272 of human Sox17 protein. Background SRY-related high mobility group box (SOX) proteins comprise a large family of widely conserved transcription factors that play important roles in development. SOX proteins possess a high mobility group (HMG) motif that binds the DNA minor groove. SOX proteins do not directly mediate transcription, but require binding partners that regulate their ability to mediate transcription of target genes that control cell fate determination and development (reviewed in 1). Transcription factor Sox17 is a Sox family protein with an established role in endoderm Specification during development (2). In addition, Sox17 plays essential roles in the maintenance of the hematopoietic stem cell pool (3) and for vascular morphogenesis during development (4,5). Sox17 negatively regulates oligodendrocyte precursor differentiation by antagonizing β-catenin signaling (6). Mutation in the corresponding gene in humans is associated with a form of vesicoureteral reflux, a disorder characterized by congenital kidney and urinary tract defects (7). Alternate Names FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-box 17; SRY-box transcription factor 17; SRY-related HMG-box transcription factor SOX17; Transcription factor SOX-17; VUR3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG