CST, 2081S, La Antigen Antibody

Polyclonal Antibody for studying LA antigen. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, La Antigen Antibody (CST #2081) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity La Antigen Antibody detects endogenous levels of total La Antigen protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys41 of human La Antigen protein. Antibodies are purified by protein A and peptide affinity chromatography. Background La antigen is recognized by antibodies in patients with autoimmune disorders such as systemic lupus erythematosus and Sjögren's syndrome (1). La antigen binds to the 5'-noncoding region of poliovirus RNA and is an IRES trans-acting factor (1,2). Depletion of La antigen reduces the function of poliovirus IRES (3). La antigen, when phosphorylated at Ser366, has been shown to associate with nuclear precursor tRNAs and facilitate their processing (4). The nonphosphorylated La antigen interacts with the mRNAs that have 5'-terminal oligopyrimidine (5'TOP) motifs to control protein synthesis (4). Alternate Names autoantigen La; La; La autoantigen; La ribonucleoprotein; La ribonucleoprotein domain family, member 3; La/SSB; LARP3; lupus La antigen; Lupus La protein; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B; Sjogren syndrome antigen B (autoantigen La); small RNA binding exonuclease protection factor La; SS-B; SS-B/La protein; SSB Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit