CST, 20944S, Whitlow/218 Linker (E3U7Q) F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying . Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, Whitlow/218 Linker (E3U7Q) F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) (CST #20944) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity Whitlow/218 Linker (E3U7Q) F(ab') Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide. Background The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1). Human F receptors exhibit strong cross-reactivity with rabbit IgG, and are expressed at high levels on cells of myeloid lineage (e.g., monocytes and myeloid-derived cell lines). Fluorescent anti-linker F(ab') fragment conjugates are ideal for use in flow cytometry assays on cells containing F receptors, and eliminate the need for an F blocking step prior to antibody incubation. Cell Signaling Technology employs strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence. Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG