CST, 2261S, Phospho-(Ser) PKC Substrate Antibody

Polyclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, Phospho-(Ser) PKC Substrate Antibody (CST #2261) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:25 Peptide ELISA (DELFIA): 1:500 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-(Ser) PKC Substrate Antibody recognizes endogenous levels of many cellular proteins only when phosphorylated at serine residues surrounded by Arg or Lys at the -2 and +2 positions and a hydrophobic residue at the +1 position. This antibody does not cross-react with nonphosphorylated serine residues, with phospho-threonine in the same motif, or with phospho-serine in other motifs. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with synthetic phospho-PKC substrate peptides. Antibodies are purified by protein A and peptide affinity chromatography. Background Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction, many PKC substrates continue to be unidentified (1,2). Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). Specification REACTIVITY: All SENSITIVITY: Endogenous SOURCE: Rabbit