CST, 2276L, Myc-Tag (9B11) Mouse Monoclonal Antibody

Monoclonal Antibody for studying . Validated for WB,WB,IP,IHC,IF,F,ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, Myc-Tag (9B11) Mouse Monoclonal Antibody (CST #2276) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:250 Immunohistochemistry (Paraffin): 1:1500 - 1:6000 Immunofluorescence (Immunocytochemistry): 1:1000 - 1:2000 Flow Cytometry (Fixed/Permeabilized): 1:500 - 1:2000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 58730 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP Specificity / Sensitivity Myc-Tag (9B11) Mouse Monoclonal Antibody detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may cross-react with c-myc protein. This antibody may weakly cross-react with a protein of unknown origin ~90kDa. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG2a kappa