CST, 23180T, Dab1 (F6E1K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Dab1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Dab1 (F6E1K) Rabbit Monoclonal Antibody (CST #23180) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Dab1 (F6E1K) Rabbit mAb recognizes endogenous levels of total Dab1 protein. This antibody does not cross-react with Dab2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Dab1 protein. Background The Reelin signaling pathway plays a critical role in neuronal development. Reelin is a secreted glycoprotein that binds to the lipoprotein receptors VLDLR and ApoER2 or alpha3beta1 integrin on the surface of neurons (1,2). Activation of these receptors induces tyrosine phosphorylation of Disabled 1 (Dab1), an intracellular adaptor. It is generally believed that tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is the most critical downstream event in Reelin signaling. The phosphotyrosine-binding (PTB) domain within its amino terminus enables Dab1 to recognize and bind to a conserved sequence motif within the cytoplasmic tail of the receptors. In addition, the PTB domain contains a pleckstrin homology-like subdomain that binds to phosphoinositides. The phosphoinositide-binding region within the Dab1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab1 independent of VLDLR and ApoER2 (3). It has been demonstrated that Src, CrkII, CrkL, and Dock1 associate with tyrosine-phosphorylated Dab1. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232 (4). Alternate Names DAB adaptor protein 1; Dab reelin signal transducer 1; Dab, reelin signal transducer, homolog 1; DAB1; DAB1, reelin adaptor protein; Disabled homolog 1; disabled homolog 1 (Drosophila); SCA37 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80, 77 Source/Isotype: Rabbit IgG