CST, 23192S, Thyroid Transcription Factor 1 (TTF-1) (SPT24) Mouse Monoclonal Antibody

Monoclonal Antibody for studying TTF-1. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Thyroid Transcription Factor 1 (TTF-1) (SPT24) Mouse Monoclonal Antibody (CST #23192) is ready to ship. Product Usage Information IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Thyroid Transcription Factor 1 (TTF-1) (SPT24) Mouse Monoclonal Antibody recognizes endogenous levels of TTF-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to a 123 amino acid fragment of the N-terminal region of human TTF-1 protein. Background Thyroid Transcription Factor 1 (TTF-1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF-1 plays an important role in differentiation and morphogenesis of the developing thyroid, lung, and ventral forebrain (2). TTF-1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF-1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6). Alternate Names BCH; BHC; Homeobox protein NK-2 homolog A; Homeobox protein Nkx-2.1; NK-2; NK-2 homolog A; NK2 homeobox 1; NKX2-1; NKX2.1; NKX21; NKX2A; NMTC1; T/EBP; TEBP; Thyroid nuclear factor 1; Thyroid transcription factor 1; Thyroid-specific enhancer-binding protein; TITF1; TTF-1; TTF1 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1