CST, 23842S, BAG3 (E7Y9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BAG3. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, BAG3 (E7Y9T) Rabbit Monoclonal Antibody (CST #23842) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity BAG3 (E7Y9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total BAG3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human BAG3 protein. Background BCL2-associated athanogene 3 (BAG3) is a member of the BAG domain-containing family of proteins important for protein homeostasis, including trafficking of misfolded proteins and the regulation of molecular chaperones, transcription factors, and autophagy (1-3). BAG3 is closely associated with molecular chaperones by interacting with the N-terminal ATPase domain of 70 kDa heat shock protein (HSP70) (4). HSP70 is a highly versatile molecular chaperone that is regulated by environmental stresses and interacts with unfolded proteins to promote proper refolding and prevent irreversible aggregation (5). BAG3 function has been associated with the regulation of apoptotic proteins and targeting polyubiquitinated client proteins for autophagic degradation (2). Expression of BAG3 is elevated in a variety of tumor types and regulates multiple aspects of cancer progression including cell adhesion, metastasis, angiogenesis, enhanced autophagic activity, and inhibition of apoptosis (2). Alternate Names BAG cochaperone 3; BAG family molecular chaperone regulator 3; BAG-3; BAG-family molecular chaperone regulator-3; BAG3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; BCL2 associated athanogene 3; BCL2-associated athanogene 3; BCL2-binding athanogene 3; BIS; CAIR-1; Docking protein CAIR-1; MFM6; MGC104307 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG