CST, 24029S, Dopamine Transporter/DAT (E3U7R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DAT mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Dopamine Transporter/DAT (E3U7R) Rabbit Monoclonal Antibody (CST #24029) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Dopamine Transporter/DAT (E3U7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total dopamine transporter/DAT protein. Non-specific labeling of mouse colon may be observed by immunofluorescence. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro26 of mouse dopamine transporter/DAT protein. Background Dopamine is a neurotransmitter that plays important roles in the brain, particularly in dopamine pathways that control the motivational component of reward-motivated behavior. These behavioral outputs are generated by the basal ganglia via its interaction with multiple brain areas that modulate sensorimotor, emotional, and cognitive information (1). The spatiotemporal dynamics of dopamine signaling are regulated by the dopamine transporter (DAT), a presynaptic transmembrane protein that drives the reuptake of dopamine released into the synaptic cleft. This process occurs in a sodium- and chloride-dependent manner, with DAT transporting two sodium cations down their concentration gradient, along with one chloride anion and the dopamine substrate. DAT is a member of the neurotransmitter sodium symporter (NSS) family, which also includes serotonin, norepinephrine, glycine, and γ-aminobutyric acid (GABA) transporters. The NSS family is part of the larger solute carrier 6 (SLC6) family of proteins, which in addition to neurotransmitters, also include transporters of amino acids, osmolytes, and energy metabolites (2-5). Mutations in the DAT gene, have been linked to Parkinson's disease (PD), infantile parkinsonism-dystonia (IPD), autism spectrum disorder (ASD), attention deficit hyperactivity disorder (ADHD), major depressive disorder (MDD), and bipolar disorder (BP) (5-8). In addition to its role in the brain, DAT is expressed by lymphocytes and monocytes in the periphery, where it modulates immune function. Altered expression levels of DAT in the periphery is also a suggested biomarker for some cancers (9-12). Alternate Names DA transporter; DAT; Dat1; dopamine transporter 1; SC6A3; Slc6a3; Sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; Solute carrier family 6 member 3 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG