CST, 2412S, PA28 gamma Antibody

Polyclonal Antibody for studying PSME3. Validated for WB,IP,IHC,IF. Highly specific and rigorously validated in-house, PA28 gamma Antibody (CST #2412) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:25 Immunofluorescence (Immunocytochemistry): 1:25 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PA28 gamma Antibody detects endogenous levels of total PA28γ. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp16 of human PA2γ. Antibodies are purified by protein A and peptide affinity chromatography. Background The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionarily conserved subunits of PA28: PA28α (PSME1), PA28β (PSME2), and PA28γ (PSME3) (1,2). PA28α and PA28β form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28α/β complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptides from foreign proteins (2). PA28γ exists in the form of a homoheptamer and is mainly located in the nucleus. The PA28γ complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4). Alternate Names 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; Activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; HEL-S-283; Ki; Ki antigen; Ki nuclear autoantigen; Ki); PA28 gamma variant 5; PA28-gamma; PA28g; PA28gamma; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Proteasome activator 28 subunit gamma; proteasome activator 28-gamma; Proteasome activator complex subunit 3; proteasome activator subunit 3; PSME3; REG gamma-3; REG-gamma Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 30 SOURCE: Rabbit