CST, 2426T, PHB1 Antibody

Polyclonal Antibody for studying PHB. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PHB1 Antibody (CST #2426) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PHB1 Antibody detects endogenous levels of total PHB1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser252 of human PHB1. Antibodies are purified by protein A and peptide affinity chromatography. Background The prohibitins PHB1 and PHB2 are highly conserved, multifunctional proteins present in eukaryotic nuclear and mitochondrial compartments. PHB1 is a 30 kDa tumor suppressor protein involved in cell cycle control (1). PHB1 has been found in mitochondria, the nucleus, and the plasma membrane, as well as extracellularly in circulation (2). In mitochondria, prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (3,4). In the nucleus, prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription (2,5). Extracellular prohibitin can bind and activate C3 to enhance complement activation (6). Alternate Names epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; HEL-215; HEL-S-54e; PHB; PHB1; Prohibitin; Prohibitin 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 29 SOURCE: Rabbit