CST, 24326S, CD16 (D1N9L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD16/FCgR3a. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD16 (D1N9L) Rabbit Monoclonal Antibody (CST #24326) is ready to ship. Product Usage Information IHC Leica Bond: 1:200 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 72204 . Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD16 (D1N9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD16 protein. This antibody detects CD16A and CD16B. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro32 of human CD16 protein. Background CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1). CD16A is expressed by NK cells, macrophages, and a subset of monocytes, while CD16B is expressed by neutrophils (4). CD16 is commonly used in combination with CD56 to characterize NK cells, with CD16 identifying NK cells capable of cytotoxicity (5). Alternate Names CD16; CD16A; CD16a antigen; Fc fragment of IgG receptor IIIa; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc gamma receptor III-A; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIIIa; FCG3; FCG3A; FcgammaRIIIA; FCGR3; FCGR3A; FCGRIII; FcR-10; FcRIII; FcRIIIa; IGFR3; IgG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; low affinity immunoglobulin gamma receptor III-a Fc fragment; neutrophil-specific antigen NA Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG