CST, 2435L, PPAR gamma (C26H12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PPAR-gamma. Validated for WB,IHC,IF,ChIP,ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, PPAR gamma (C26H12) Rabbit Monoclonal Antibody (CST #2435) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:200 Chromatin IP: 1:100 Chromatin IP-seq: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq Specificity / Sensitivity PPAR gamma (C26H12) Rabbit Monoclonal Antibody detects endogenous levels of total PPARγ protein. Non-specific labeling may be observed by immunofluorescence in mitotic cells. Species Reactivity: Human, Mouse Source / Purification PPARγ (C26H12) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Asp69 of human PPARγ. Background Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5). Alternate Names CIMT1; GLM1; NR1C3; Nuclear receptor subfamily 1 group C member 3; peroxisome proliferative activated receptor gamma; peroxisome proliferator activated receptor gamma; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing; PPAR gamma; PPAR-gamma; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 53, 57 Source/Isotype: Rabbit IgG