CST, 24442S, CD11b/ITGAM (M1/70) Rat Monoclonal Antibody (FITC Conjugate)

Monoclonal Antibody for studying CD11b/ITGAM. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD11b/ITGAM (M1/70) Rat Monoclonal Antibody (FITC Conjugate) (CST #24442) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.5 μg of antibody per test. Flow Cytometry (Fixed/Permeabilized): 1:100 Flow Cytometry (Live): 1:100 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD11b/ITGAM (M1/70) Rat Monoclonal Antibody (FITC Conjugate) recognizes endogenous levels of total CD11b protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Human, Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4). The M1/70 antibody is widely used as a marker for CD11b expression on neutrophils, macrophages, NK cells, and dendritic cells (DC) (5,6). Alternate Names antigen CD11b (p170); CD11 antigen-like family member B; CD11B; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; Integrin alpha-M; integrin subunit alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITAM; ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2b kappa