CST, 2458S, PSMA5 (T14) Antibody

Polyclonal Antibody for studying PSMA5. Validated for Western Blotting. Highly specific and rigorously validated in-house, PSMA5 (T14) Antibody (CST #2458) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PSMA5 (T14) Antibody detects endogenous levels of total PSMA5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr14 of human PSMA5 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of α subunits (called PSMAs), and the two inner layers are identical rings composed of β subunits. While the catalytic sites are located on the β rings (1-3), the α subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different α and ten different β proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades polyubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9). Alternate Names epididymis tissue sperm binding protein Li 11n; macropain subunit zeta; Macropain zeta chain; MGC117302; MGC125802; MGC125803; MGC125804; Multicatalytic endopeptidase complex zeta chain; proteasome (prosome, macropain) subunit, alpha type, 5; proteasome 20S subunit alpha 5; proteasome alpha 5 subunit; proteasome component 5; proteasome subunit alpha 5; Proteasome subunit alpha type-5; proteasome subunit zeta; Proteasome zeta chain; PSA5; PSC5; PSMA5; ZETA Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 27 SOURCE: Rabbit