CST, 2461T, Phospho-Rac1/cdc42 (Ser71) Antibody

Polyclonal Antibody for studying Rac1 (Ser71) phosphate/Cdc42 (Ser71) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Rac1/cdc42 (Ser71) Antibody (CST #2461) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Rac1/cdc42 (Ser71) Antibody detects endogenous levels of Rac1/cdc42 only when phosphorylated at serine 71. This antibody may also recognize phospho-RhoA (Ser73). Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser71 of human Rac1/cdc42. Antibodies are purified by protein A and peptide affinity chromatography. Background Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3). A putative Akt phosphorylation site at Ser71 of Rac1/cdc42 has been identified and confirmed by in vitro kinase assay (4). Phosphorylation at this site may inhibit GTP binding of Rac1, attenuating the signal transduction pathway downstream of Rac1 (4). Alternate Names CDC42; CDC42Hs; Cell division control protein 42 homolog; cell division cycle 42; cell division cycle 42 (GTP binding protein, 25kDa); Cell migration-inducing gene 5 protein; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K; G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; GTP-binding protein, 25kD; MGC111543; MIG5; migration-inducing gene 5; MRD48; p21-Rac1; Rac family small GTPase 1; Rac-1; RAC1; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); rho family, small GTP binding protein Rac1; small GTP binding protein CDC42; TC-25; TC25; TKS Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit