CST, 2470T, PTCH2 (G1191) Antibody

Polyclonal Antibody for studying PTCH2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PTCH2 (G1191) Antibody (CST #2470) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PTCH2 (G1191) Antibody detects transfected levels of PTCH2 protein. This antibody does not recognize transfected levels of human PTCH1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region (predicted to be intracellular) surrounding residue Gly1191 of human PTCH2. Antibodies are purified by protein A and peptide affinity chromatography. Background Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10). Alternate Names patched 2; patched homolog 2; patched homolog 2 (Drosophila); Protein patched homolog 2; PTC2; PTCH2 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 130 SOURCE: Rabbit