CST, 24748SF, GATA-2 (E9T6F) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GATA2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, GATA-2 (E9T6F) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #24748) is ready to ship. Product Usage Information This product is the carrier free version of product #79802. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 79802 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GATA-2 (E9T6F) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total GATA-2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human GATA-2 protein. Background The zinc finger transcription factor GATA-2 is widely expressed and plays an essential role in many developmental processes (1). Studies on GATA-2 knockout mice indicate that this protein is required in hematopoiesis (2). GATA-2 also inhibits the differentiation of white (3) and brown adipocytes (4) and has been shown to suppress the proliferation of neuronal progenitor cells (5). Alternate Names DCML; Endothelial transcription factor GATA-2; FLJ45948; GATA binding protein 2; GATA-binding protein 2; GATA2; IMD21; MGC2306; MONOMAC; NFE1B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 51 Source/Isotype: Rabbit IgG