CST, 25048S, PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying PD-L1. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #25048) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 Flow Cytometry (Live): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with mammalian cells expressing full length PD-L1 protein. Background Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor-infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9). Alternate Names B7 homolog 1; B7-H; B7-H1; B7H1; CD274; CD274 antigen; CD274 molecule; hPD-L1; MGC142294; MGC142296; PD-L1; PD1L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell death 1 ligand 1; Programmed death ligand 1 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG