CST, 25792S, SOX5 (E8S4A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SOX5. Validated for Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SOX5 (E8S4A) Rabbit Monoclonal Antibody (CST #25792) is ready to ship. Product Usage Information For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Chromatin IP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Chromatin IP Specificity / Sensitivity SOX5 (E8S4A) Rabbit Monoclonal Antibody detects endogenous levels of SOX5 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human SOX5 protein. Background SOX5 is a member of the SOX (SRY-related HMG-box) family of transcription factors. It is involved in the regulation of embryonic development, and specifically essential for driving chondrogenesis (1). In recent years, it has become apparent that SOX5 plays a significant role in the progression of various tumor types, such as breast cancer, glioma, hepatocellular carcinoma, lung cancer, and others (2-5). Upregulation of SOX5 in these tumor types has been reported to drive metastasis through activation of epithelial-mesenchymal transition (EMT) (2-5). Alternate Names L-SOX5; L-SOX5B; L-SOX5F; LAMSHF; MGC35153; SOX5; SRY (sex determining region Y)-box 5; SRY-box 5; SRY-box transcription factor 5; Transcription factor SOX-5 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG