CST, 2760S, Glucagon Antibody

Polyclonal Antibody for studying Glucagon. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Glucagon Antibody (CST #2760) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Glucagon Antibody detects endogenous levels of total glucagon protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr62 of human glucagon protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Glucose homeostasis is regulated by a variety of hormones, including glucagon. Glucagon is synthesized as the precursor molecule proglucagon and is proteolytically processed to yield the mature peptide in α cells of the pancreatic islets. Glucagon causes the release of glucose from glycogen and stimulates gluconeogenesis in the liver (1,2). Alternate Names GCG; Glicentin; Glicentin-related polypeptide; GLP-1; GLP-1(7-36); GLP-1(7-37); GLP-2; GLP1; GLP2; GLUC; Glucagon; Glucagon-like peptide 1; Glucagon-like peptide 1(7-36); Glucagon-like peptide 1(7-37); Glucagon-like peptide 2; GRPP; Incretin hormone; OXM; OXY; Oxyntomodulin; preproglucagon; Pro-glucagon Specification REACTIVITY: H M R SENSITIVITY: Endogenous SOURCE: Rabbit