CST, 2791S, Phospho-Tyrosine Hydroxylase (Ser40) Antibody

Polyclonal Antibody for studying Tyrosine Hydroxylase (Ser71) phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Phospho-Tyrosine Hydroxylase (Ser40) Antibody (CST #2791) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-Tyrosine Hydroxylase (Ser40) Antibody detects endogenous levels of tyrosine hydroxylase only when phosphorylated at serine 40. Species Reactivity: Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Ser40 of human tyrosine hydroxylase. Antibodies are purified by protein A and peptide affinity chromatography. Background Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and post-translational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31, and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9). Alternate Names dystonia 14; DYT14; DYT5b; HTH-2; TH; TH2; TY3H; TYH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; tyrosine hydroxylase Specification REACTIVITY: R SENSITIVITY: Endogenous MW (kDa): 55-60 SOURCE: Rabbit