CST, 28182S, CD72 (E4K4A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD72. Validated for Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, CD72 (E4K4A) Rabbit Monoclonal Antibody (CST #28182) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity CD72 (E4K4A) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD72 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln314 of human CD72 protein. Background CD72 is an inhibitory co-receptor of the B cell antigen receptor (BCR) and is expressed primarily on B cells (1,2). The cytoplasmic domain of CD72 contains immunoreceptor tyrosine-based inhibition motifs (ITIMs) that are phosphorylated by Lyn when Lyn associates with BCR (3-5). This phosphorylation leads to recruitment of SHP-1 to CD72 and negative regulation of BCR signaling (4,5). The extracellular portion of CD72 contains a C-type lectin domain, and binding its ligand, CD100/Semaphorin-4D, leads to dissociation of CD72 and SHP-1 and thus relief of BCR signaling suppression (6,7). Recently, Semaphorin-3A was also reported to act as a ligand for CD72; however, unlike CD100/Semaphorin-4D, association with Semaphorin-3A induces CD72 ITAM phosphorylation and association of CD72 with SHP-1 (8). CD72 also plays a role in systemic lupus erythematosus (SLE); through its recognition of the self-antigen Sm/RNP, CD72 suppresses the TLR7-dependent B cell response to nucleic acid self-antigens, inhibiting SLE development (9). Alternate Names B-cell differentiation antigen CD72; CD72; CD72 antigen; CD72 molecule; CD72b; Lyb-2; LYB2 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG