CST, 2849S, beta-Catenin (L54E2) Mouse Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying Catenin-beta. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, beta-Catenin (L54E2) Mouse Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #2849) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:25 - 1:100 Immunofluorescence (Immunocytochemistry): 1:25 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity beta-Catenin (L54E2) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β-catenin. The antibody was conjugated to Alexa Fluor 488 under optimal conditions with an F/P ratio of 2-5. Background β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). Alternate Names armadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV Specification REACTIVITY: H R SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1